Genome Integrity

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 1  |  Page : 1-

Analysis of Xq27.3 fragility using the micronucleus-fluorescence In situ hybridization assay


Leyla Ozer, Hatice Ilgin Ruhi, Isik Bökesoy 
 Mikrogen Genetic Diagnosis Center; Division of Medical Genetics, Faculty of Medicine, Ankara University, Ankara, Turkey

Correspondence Address:
Leyla Ozer
Cinnah Caddesi 47/1, Cankaya, Ankara
Turkey

Chromosome fragile sites tend to form gap or break in chromosomes following when the cell exposed to replication stress. Folic acid deprivation in the culture medium induces folate-sensitive rare fragile sites, such as FRAXA which is responsible for the fragile X mental retardation syndrome. Chromosome instability at fragile sites can be evaluated by biomarkers of genomic instability such as frequency of micronuclei (MN). It was aimed to analyse the chromosome content of MN in Fragile X cells during folate deprivation by the MN-fluorescence in situ hybridization (FISH) method. Samples from five Fragile X syndrome patients, diagnosed using cytogenetic and molecular methods, as well as from their parents and five controls were included in the study. Blood samples were cultured in two different culture media (folate-deficient and normal). Results of MN-FISH test were analysed in terms of MN frequency and chromosome content of MN. An accumulation of MN in Fragile X patients, mainly containing T (+) or C (+) MN or T (+) plus C (+) MN in binucleated cells was found. Finally, MN-FISH analysis allowed confirming that the increase in MN frequency is due to a higher sensitivity to chromosome breakage along the X chromosome.


How to cite this article:
Ozer L, Ruhi HI, Bökesoy I. Analysis of Xq27.3 fragility using the micronucleus-fluorescence In situ hybridization assay.Genome Integr 2020;11:1-1


How to cite this URL:
Ozer L, Ruhi HI, Bökesoy I. Analysis of Xq27.3 fragility using the micronucleus-fluorescence In situ hybridization assay. Genome Integr [serial online] 2020 [cited 2020 Sep 26 ];11:1-1
Available from: http://www.genome-integrity.org/article.asp?issn=2041-9414;year=2020;volume=11;issue=1;spage=1;epage=1;aulast=Ozer;type=0