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 Table of Contents  
ORIGINAL ARTICLE
Year : 2021  |  Volume : 12  |  Issue : 1  |  Page : 1-10

Association of telomere length and serum vitamin D levels with type 2 diabetes mellitus and its related complications: A possible future perspective


1 Department of General Medicine, Jawaharlal Nehru Medical College, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India
2 Department of Kayachikitsa (General Medicine), Alva's Ayurveda Medical College, Moodbidri, Karnataka, India
3 Dr. Prabhakar Kore Basic Science Research Centre, Belagavi, Karnataka, India
4 Department of Biochemistry, USM-KLE, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India
5 Department of Ophthalmology, Jawaharlal Nehru Medical College, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India

Date of Web Publication18-Nov-2021

Correspondence Address:
C Akash
Department of General Medicine, Jawaharlal Nehru Medical College, KLE Academy of Higher Education and Research, Belagavi - 590 010, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/genint.genint_3_21

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  Abstract 


Evidence show that shortened telomere length (TL) and low Vitamin D levels can increase the risk of type 2 diabetes mellitus (T2DM) and its associated complications. T2DM has been considered as an age-related disease, it may be associated with TL. The study aimed to evaluate the association of TL and Vitamin D levels with complications of T2DM and the impact of Vitamin D on TL in patients with T2DM. This 1-year cross-sectional study was conducted at a tertiary care hospital on 90 patients. Height, weight, body mass index, waist-hip ratio was calculated. Fasting blood sugars, postprandial blood sugar, and glycated hemoglobin (HbA1c) were analyzed. Absolute TL was obtained from quantitative real-time polymerase chain reaction (qPCR). Vitamin D estimation was done by chemiluminescent immunoassay. Descriptive analysis of the data was done using R i386 3.6.3. The study found a positive correlation between TL and Vitamin D levels (r = 0.64; P < 0.0001). The interaction with high HbA1c levels and lower levels of Vitamin D led to the shortening of TL (P = 0.0001). The median of TL and mean of Vitamin D levels were significantly less in the diabetic group (P < 0.0001). Vitamin D levels positively affected the TL and its levels had an inverse relation with the HbA1c levels. This association had a significant effect on the shortening of TL. Vitamin D also had a significant association with other diabetic complications that instigated the shortening of TL. Therefore, assessing the role of Vitamin D levels on the shortening of TL can prove to be crucial biomarkers in managing optimal glycemic levels in T2DM patients.

Keywords: Anthropometry, glycated hemoglobin, telomere length, type 2 diabetes mellitus, Vitamin D


How to cite this article:
Akash C, Prabhu M, Maldar A, Akash P, Mishra S, Madhura T K, Kumar S, Patil RS, Piplani S, Smitha K S. Association of telomere length and serum vitamin D levels with type 2 diabetes mellitus and its related complications: A possible future perspective. Genome Integr 2021;12:1-10

How to cite this URL:
Akash C, Prabhu M, Maldar A, Akash P, Mishra S, Madhura T K, Kumar S, Patil RS, Piplani S, Smitha K S. Association of telomere length and serum vitamin D levels with type 2 diabetes mellitus and its related complications: A possible future perspective. Genome Integr [serial online] 2021 [cited 2021 Nov 27];12:1-10. Available from: https://www.genome-integrity.org/text.asp?2021/12/1/1/330585




  Background Top


Telomeres present at the chromosomal ends are made of repetitive nucleotide sequences. They are often considered as biomarkers for biological ageing.[1] Telomere length (TL) varies due to genetic, environmental factors, cancer, and various age-related diseases like cardiovascular disease, type 2 diabetes mellitus (T2DM).[1],[2] Among the chronic diseases, T2DM is one of the most prevalent diseases globally with a prediction that by the year 2030 around 483 million individuals might get affected.[1],[3] Since T2DM has been considered an age-related disease, it may be associated with TL. Evidence have established that there is shortened TL in patients with T2DM, while others have shown a negative co-relation of TL and T2DM.[2],[3] It has been reported that the levels of Vitamin D affect the mechanisms of insulin secretion and insulin sensitivity in T2DM patients. Besides this, it is considered an important marker for predicting glycemic levels.[4] Furthermore, the levels of Vitamin D could affect the TL mechanisms by decreasing the cell proliferation and inflammation that affect the total count of leukocytes and further attrition of leukocyte TL.[5],[6],[7] Vitamin D is involved in many metabolic processes such as cellular differentiation, apoptosis, cell proliferation, and many more, as a result of such multiple functions of Vitamin D, it can be predicted that it is highly associated with cellular ageing and telomere biology.[8] It has been reported that high Vitamin D levels and longer TL can reduce the risk of chronic diseases like T2DM and its progression. Another aspect is the increased levels of oxidative stress and inflammation may lead to reduction of telomerase activity and increase the telomere shortening which are the main feature in obese individuals.[8] According to a study, it was seen that those with the lowest quartile of omega-3 fatty acids undergo accelerated telomere shortening. In such cases, Vitamin D supplements play a vital role in correcting the TL activity in obese individuals. Since Vitamin D is associated with a range of vital cellular processes impacting cellular ageing and telomere biology its role needs to be determined.[6],[9] Hyperglycemia-induced activation of polyol pathway exhausts NADPH, depleting the supply of reducing equivalents and synthesis of reduced glutathione.[10]

Hence, the present study aimed to evaluate the association of TL and Vitamin D levels in T2DM and its related complications and to find the impact of Vitamin D on TL as well. Further, the study also attempted to find its associations with parameters such as physical activity, body mass index (BMI), waist-hip ratio (WHR), diabetic complications, etc., in this subset of patients.


  Methods Top


A 1 year cross-sectional study was conducted at a tertiary care hospital and medical research centre in South India. The study is approved by the Institutional ethics committee (No. MDC/DOME/50). Written informed consents were obtained from the participants. A total of 90 patients were included and subdivided into two groups based on symptoms, fasting blood sugars (FBS), postprandial blood sugar (PPBS), and glycated hemoglobin (HbA1c) as diabetic and nondiabetic (normal). Inpatient or outpatient T2DM with any duration of disease on treatment with oral hypoglycemic agents or insulin (n = 45) and nondiabetic (n = 45) patients above the age of 18 years were included in the study. None of the patients were on Vitamin D supplements. Patients with Type 1 DM, pancreatic disorders, or who had diabetes secondary to other systemic diseases or known cases of chronic inflammatory or infectious diseases based on good clinical history were excluded from the study. Demographic data and detailed history were recorded. A thorough physical examination included the recording of vital data (pulse rate, blood pressure, temperature, and respiratory rate), general physical and systemic examination were done. The anthropometric measurements such as height, weight, BMI, and WHR of each patient in the study were systematically measured, calculated, and documented.

The FBS, PPBS, and HbA1c levels were measured and documented as per the diagnostic criteria given by the American Diabetes Association, 2020.[11] FBS and PPBS were measured by using strip glucometer. HbA1c was estimated by high-performance liquid chromatography method. The absolute TL of each subject was obtained by qPCR. The procedure followed is explained below. For sample size calculation below formula was used,

n = (z_[1− α/2] + z_[1−β])^2 ([τ + γ] σ_1^2]/(γ [μ_1− μ_2]^2) + ([τ^2+ γ^3] z_[1− α/2]^2)/(2 γ(ைτ+ γ)௉^2)

n = the sample size required for each group,

τ = (σ_2^2)/(σ_1^2),

γ =1,

β = power of the test,

α = statistical significance level.

where σ_0^2 and σ_1^2 are variances of two groups.

In a study, it was found that mean TL in T2DM was 6.01 kb ± 0.2, and in controls, it was 9.11 ± 0.6.[12]

Hence, the α is taken to be 0.05 and power β =0.90. Here, τ = (σ_2^2)/(σ_1^2) =8.998089 ≈ 9. Therefore, n = (1.96 + 1.645)^2× ((9 + 1)ை1.78௉^2)/(1 [9.11 − 6.01]^2) + ([9^2 + 1^3]ை1.96௉^2)/2 × 1(ை1 + 9)௉^2, n = 45. Hence, for each group at least 45 subjects are needed for the study. The above sample size is representative of the same.

DNA sample collection, extraction, amplification, and measurement

About 3 ml of venous blood sample was collected from the subjects participating in the study in an EDTA bulb,[13],[14] and processed immediately for DNA isolation and for assessing the TL by Real-time polymerase chain reaction (PCR) as per the standard procedure. The Qiagen DNA Mini Kit (QIAGEN Inc., California, United States Lot no. 163032024 manufactured in 2019) was used to extract the DNA from the peripheral blood leukocytes.[15] After the DNA was isolated its TL was measured using quantitative RT-PCR as T/S ratio. Telomere standard curve was generated by plotting CT (cycle threshold) values against the amount of telomere sequence in kb per reaction [Figure 1]. Cycling conditions for both the Telomere and 36B4 amplicons were as follows: 10 min at 95°C followed by 40 cycles at 95°C for 15s and 60°C for 1 min, followed by a melt curve. The 36B4 is a Single-Copy Gene and serves as a reference gene in the conventional qPCR technique in the measurement of Telomeres [Figure 2].[14] All samples were analysed in the ABI Step One Plus RT-PCR System with SDS version Step One Plus software.[13]
Figure 1: Standard curve for polymerase chain reaction cycles (generated by plotting cycle threshold values against the amount of telomere sequence in kb per reaction)

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Figure 2: Single copy gene standard curve

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Chemiluminescence immunoAssay for Vitamin D

DiaSorin LIAISON® 25 Hydroxy Vitamin D TOTAL Assay 100 kit (test code 310600), was used to assess the Vitamin D levels in our patients. The test was advanced chemiluminescence technology with magnetic microparticles. One hundred μL standard or sample was added to each well and then incubated for 90 min at 37°C. The liquid is then removed. Following which 100 μL Biotinylated detection Ab was added and then incubated for 1 h at 37°C. The fluid was aspirated and washed thrice. One hundred μL avidin-horseradish peroxidase Conjugate was added and incubated for 30 min at 37°C. Fluid is aspirated and washed 5 times. One hundred μL of substrate mixture solution was added and incubated for 5 min at 37°C. Readings were immediately noted after the substrate reaction time and results were calculated to obtain the Vitamin D levels.[16]

Statistical analysis

Descriptive analysis of the data was performed using R i386 3.6.3. Continuous variables were represented by mean ± standard deviation form and compared using t-test, Welch t-test, Mann–Whitney U-test, ANOVA, Kruskal–Wallis test. Categorical variables were represented by frequency tables and compared using the Chi-square test, Cochran Armitage test. P < 0.05 was considered as significant.


  Results Top


A total of 90 patients were considered in the study, with a subdivision of 45 diabetic and 45 nondiabetic. The mean age of the participants was 58.29 ± 13.87 (in years). One-tailed Welch t-test shows that the mean BMI of diabetic subjects is significantly more than nondiabetic subjects (P < 0.0001), odds ratio [OR] 4.59 (95% confidence interval CI: [2.67, 9.64]). Using one-tailed Mann–Whitney U-test; the median of WHR was significantly more in diabetic subjects than nondiabetic patients (P = 0.0006), OR 7.47 (95% CI:[2.65,24.15). The Cochran Armitage test showed a significant linear decreasing trend in the proportion of diabetes over physical activity. The chances of developing T2DM if the patient exercised regularly, exercised sometimes and who never exercised was 20.0%, 30.77%, and 69.39%, respectively [Figure 3] and [Table 1].
Figure 3: Prevalence of diabetes over physical activity

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Table 1: Demographic detail of the study participants

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Cerebrovascular disease (CVD) (P = 0.0185) and coronary artery disease (CAD) (P < 0.0001) are significantly associated with Diabetes. The odds ratio (OR) of CVD and CAD for diabetic patients is 14.96 (95% CI: [1.04, 214.57), 9.80 (95% CI: [3.01,31.95]), respectively, times higher than nondiabetic patients [Figure 4] and [Table 2].
Figure 4: Comparison of complications (cerebrovascular disease, coronary artery disease) with diabetes status (in both diabetic and nondiabetic groups)

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Table 2: Association of diabetes and complications

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TL is significantly less in diabetic group (P < 0.0001). The mean TL in diabetic and nondiabetic being 87.71 ± 76.07 and 548.58 ± 329.62 kb/diploid genome, respectively. Using one tailed t-test, it has been concluded that the mean of Vitamin D was significantly less in diabetic patients (P < 0.0001), odds 1.12 (95% CI: [1.06, 1.18]). Using Chi-square test, it is concluded that there is a significant association between elevated creatinine and diabetes. The odds of elevated creatinine is 10.75 (95% CI: [2.29, 50.51) times higher in diabetic patients [Table 3].
Table 3: Comparison of clinical investigation between diabetic and nondiabetic subjects

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There is a significant interaction of HbA1c levels and Vitamin D levels on TL (P < 0.0001) [Table 4].
Table 4: Relationship between glycated hemoglobin, Vitamin D with telomere length

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Using Spearman rank correlation, it has been concluded that there is a strong positive correlation between TL and Vitamin D (rho = 0.64; P < 0.0001) [Figure 5].
Figure 5: Relationship between glycated hemoglobin and Vitamin D with telomere length (depicting the impact of diabetes mellitus [represented by glycated hemoglobin] and Vitamin D levels on the telomere length)

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The mean Vitamin D in the diabetic patients who did physical activity regularly and occasionally was significantly different from diabetic patients who never did physical activity (P < 0.0001). The median of TL in the diabetic patients with CAD (P = 0.0010) was significantly less, odds 1.01 (95% CI: [1.01, 1.02]), and the mean Vitamin D level was significantly less for the diabetic subjects with CAD (P < 0.0001), odds 1.28 (95% CI: [1.16, 1.46]) than patients without CAD. The mean of TL and Vitamin D was significantly less in diabetic retinopathy (DR) patients among diabetic patients, than those without DR (P < 0.0001), odds 1.03 (95% CI: [1.01, 1.06]) and (P = 0.0030), odds 1.19 (95% CI: [1.06, 1.38]), respectively [Table 5].
Table 5: Comparison of telomere length and Vitamin D over different factors in diabetic subjects

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TL was negatively correlated with BMI (rho = −0.39; P = 0.0083) and WHR (rho = −0.28; P = 0.0332) in diabetic patients. There was a significant high negative correlation between TL and parameters of HbA1c (rho = −0.99; P < 0.0001), FBS (rho = −0.97; P < 0.0001), PPBS (rho = −0.98; P < 0.0001) in diabetic group. Vitamin D levels was negatively correlated with BMI (r = −0.25; P = 0.0470), duration of diabetes mellitus (−0.32; P = 0.0165), and serum creatinine (r = −0.31;P = 0.0381) [Table 6].
Table 6: Correlation of clinical investigations with telomere length and Vitamin D in diabetic subjects

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The mean HbA1c for CAD subjects were significantly more than normals (without CAD) in both diabetic (P = 0.01) and nondiabetic group (P = 0.02). The median of HbA1c for subjects with DR in diabetic group was significantly more than patients without DR (P = 0.0104) [Figure 6]. Using Pearson correlation coefficient, it is concluded that BMI was significantly positively correlated with HbA1c in diabetic group (r = 0.38; P = 0.0107). There was a significant strong positive correlation of HbA1c with FBS (r = 0.98; r = 0.58), PPBS (r = 0.98; r = 0.62) in both groups (P < 0.0001) [Figure 7] and [Table 7].
Figure 6: Relationship of glycated hemoglobin with complications such as cerebrovascular disease, coronary artery disease, and diabetic retinopathy in both diabetic and nondiabetic group

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Figure 7: Relationship of glycated hemoglobin with body mass index, fasting blood sugars, and postprandial blood sugar in the diabetic and nondiabetic group

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Table 7: Relationship of glycated hemoglobin with other factors in both groups

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Using one-tailed t-test, it was concluded that the mean of Vitamin D was significantly less for patients on insulin treatment than oral treatment in the diabetic group (P = 0.0044), odds 1.20 (95% CI: [1.07, 1.41]) [Table 8].
Table 8: Comparison of glycated hemoglobin, telomere length, and Vitamin D with the treatment of diabetes mellitus

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  Discussion Top


Diabetes is depicted by elevated blood glucose levels, whereas the TL is used as a biological marker of cell ageing. Clinical evidence shows a significant co-relation between T2DM and TL.[17] Hence, the current study aimed to find the association of TL and Vitamin D in T2DM and the impact of Vitamin D on the TL in patients withT2DM.

This study found that Vitamin D levels were proportional to the TL i.e. higher the Vitamin D levels (Vitamin D levels in the sufficiency range) longer the telomeres. The present study also found co-relation of Vitamin D and HbA1c levels and its effect on the TL. Higher HbA1c and lower Vitamin D levels lead to increased pace in TL shortening. This is the uniqueness of the study to find such associations in T2DM patients. According to this study, individuals with a normal to the high level of Vitamin D, the TL shortening was slower compared to those with low levels of Vitamin D.

Several metabolic factors such as obesity, insulin resistance or physical inactivity have been associated with shortened telomeres, which was also reported by Baltzis et al.;[18] after assessing telomerase activity in diabetic patients having or not, foot ulcer, on (n = 58) nonulcer group and (n = 32) ulcer group. Their study concluded that the patients with diabetic foot had significantly greater waist circumference and neuropathy disability scores along with exhibiting lower telomerase activity, indicating the possible existence of a common clinical profile among ulcer-bearing diabetic patients.

In our study, anthropometric variables like BMI and WHR showed a significant difference in the diabetic group than the nondiabetic group, which was in concordance to study by Vasanthakumar and Kambar. This indicated that the patients were obese that directly links with poor control of blood glucose levels among individuals with T2DM, while WHR is also a good predictor of visceral adipose tissue.[19] Further, TL was negatively correlated with BMI (r = −0.39; P = 0.0083) and WHR (r = −0.28; P = 0.0332) in diabetic subjects. This concurs with the exploration by Lee et al. where the BMI and WHR were inversely associated with TL (P < 0.05).[20]

Findings from another study showed a higher prevalence of diabetes in those who were less physically active (P < 0.001) similar to our findings where the majority (75.5%) of the patients who were never involved in physical activity were significantly higher in the diabetic group showing a significant corelation between the groups (P < 0.0001).[21] The study by Arsenis et al. demonstrated that exercise duration was inversely correlated with biomarkers of DNA damage and TL. Exercise mobilizes fat and enhances the detoxification process reducing oxidative stress, preserving DNA and telomeres.[22]

There was the significant mean difference between HbA1c and FBS between both diabetic and nondiabetic groups (P < 0.0001). A study by Dave et al.[23] demonstrated the significance of HbA1c and FBS among three different groups of diabetic patients (P < 0.001). This showed that level of HbA1c linearly associated with the abnormal glucose level. Values of PPBS was also significantly different between diabetics and nondiabetics (P < 0.0001). The TL in diabetics and nondiabetic individuals kb/diploid genome (P < 0.0001) showing gross shortening of telomere in T2DM in accordance with the study by Piplani et al., where the TL shortening was significantly higher in the T2DM patients (P = 0.00019).[24] The creatinine levels were highly elevated in the diabetic group than the nondiabetics (P = 0.0015). This result is supported by a study where raised serum creatinine was observed more in the diabetic patients (15/100) than the controls (2/100).[25] Vitamin D levels were significantly lower in the diabetic patients than the nondiabetics (P < 0.0001) which corroborates with a study by Kostoglou-Athanassiou et al.[26] The mean Vitamin D of diabetic patients was significantly different in those who regularly and occasionally did physical activity from those diabetic patients who were never involved in the physical activity (P < 0.0001).

A study by Kavadar et al.[27] found statistically significant difference for Vitamin D levels and a low physical activity (PA) in the insulin-resistant patients (P = 0.001). The study has thrown light on the influence of physical activity and a normal Vitamin D level on insulin levels by reducing its resistance, which can promote weight loss and prevent T2DM. Also, physical activity increases Vitamin D levels by increasing lipolysis.[27]

It was observed that the median of TL for diabetic subjects with CAD was significantly less than those without CAD (P < 0.0010) supporting the results of Xu et al. where the shorter TL was highly associated in the coronary heart disease patients than the control (P < 0.0001).[28] It was also found that the mean of TL was significantly shorter (P < 0.0001) in diabetic patients with DR, than those without it. These findings are in conformation with the study by Sharma et al. where the TL was shorter in different types of DR patients compared to the healthy controls (P = 0.0001).[29] The mean of Vitamin D was significantly less in DR patients in the diabetic patients (P = 0.0030). Similar observations were noticed in a study by Luo et al., a meta-analysis with fifteen observational studies involving 17,664 subjects, showed that low serum Vitamin D levels are associated with an increased risk of DR among T2DM patients.[30]

The duration of T2DM was moderately significant and negatively correlated with Vitamin D levels (r = −0.32; P = 0.0165) among the diabetic patients. Similar results were obtained by Alaidarous et al., who studied the impact of duration of T2DM on Vitamin D levels in 92 patients aged between 20 and 60 years. Their results concluded that T2DM duration was an independent predictor of Vitamin D deficiency.[31]

Parameters such as HbA1c (r = −0.99), FBS (r = −0.97), PPBS (r = −0.98) showed a significant negative co-relation with the TL among the diabetic patients (P < 0.0001). In a cross-sectional observational study by Dudinskaya et al., the high-risk population from Moscow, Russia between 2012 and 2013 on 99 patients (33 males and 66 females) who previously underwent an outpatient examination in the FGBI National Research Centre for Preventive Medicine showed that the parameters of FBS and HbA1c were negatively co-related with TL; however, only HbA1c showed a significant co-relation (P = 0.03).[32] The level of serum creatinine level showed a significant negative co-relation with both TL (rho = −0.05; P = 0.73) and Vitamin D levels (r = −0.31; P = 0.03) but was significantly associated with the Vitamin D levels. Matching to the results of Kumarchandra et al., where significant negative correlation between Vitamin D and serum creatinine was found (r = −0.49; P = 0.013).[33]

It was also observed that the mean HbA1c in the diabetic patients with CAD was significantly more than those diabetic patients without CAD and also with the nondiabetic patients (P > 0.05) in accordance with a study by Rezende et al. and Ul-Haque et al.[34],[35] Further, the HbA1c level was positively co-related with BMI in diabetic patients (r = 0.38; P = 0.0107) in line with the findings by Babikr et al.[36] Moreover, correlation was similar and significance between HbA1c and FBS, PPBS between both the groups (P < 0.0001) was with a higher r-value for PPBS than the FBS in the nondiabetic group. A study showed better and stronger co-relation between PPBS and HbA1c than FBS and vice versa. However, it is strongly opined that PPBS can contribute in the overall control of hyperglycemia than FBS alone.[37]

Factors such as TL, Vitamin D levels, and HbA1c were compared with the diabetic treatment (oral and insulin). It was found that vivitamid levels were significantly less in the patients receiving insulin treatment than those receiving oral treatment (P = 0.004). It is evident that people with diabetes have low levels of Vitamin D due to a weaker beta-cell function. This is concurred by Mauss et al. with the results that severe Vitamin D deficiency (<10 ng/ml) was associated with increasing FBS (β 3.13; 95% CI: 0.78, 5.47; P ≤ 0.01) and HbA1c.[38]

This study provides us strong evidence of the association between Vitamin D and TL with T2DM and its related complications, which is one of its kinds. The study design is new and unique where the relation of TL and Vitamin D levels in diabetes is sought, especially in Indian population where T2DM is an epidemic. Diabetes is progressing epidemically, so there is a need to discover newer mechanisms that govern it, to propose the latest modalities to prevent and treat diabetes. Based on anthropometric parameters obesity was related to TL. In addition, this is the first study that found an association between HbA1c levels and Vitamin D levels on TL. In future, the study can be extended to find the data about the association of TL and risk association of single nucleotide polymorphism rs2853669 with T2DM in the Indian population and finding the co-relation between total Vitamin D metabolites to predict the T2DM related complications.


  Conclusion Top


The study justified its objective to find an association of TL and Vitamin D with T2DM. Vitamin D was significantly less in the diabetic group, which was supported by higher BMI, and low physical activity in the same group. Vitamin D levels were proportional to the TL, as increased telomerase activity is noticed in those with higher levels of Vitamin D. Vitamin D levels positively affected the TL and its levels had an inverse relation with the HbA1c levels. This interaction had a significant effect on the shortening of TL. Vitamin D also had a significant association with other diabetes-related problems that instigated shortening of TL. TL was significantly shorter in the diabetic subjects. Hastened TL shortening and very low Vitamin D levels were observed in those diabetic patients having CAD and DR. The anthropometric parameters such as BMI, WHR, and the parameter serum creatinine were negatively correlated with TL in diabetic patients. A higher HbA1c levels was associated with low levels of Vitamin D, which together was further responsible for the shortening of TL. Therefore, supplementation of Vitamin D or intake of Vitamin D-rich food, optimum physical activity can not only help in correcting the deficiency but can help in achieving better and optimum glycemic control. This will further have a positive impact on the telomerase activity, by controlling telomere attrition and slowing down the shortening of TL in this subset of the population. Overall, correcting the Vitamin D levels can positively improve the maintenance of telomere, which will slower cell ageing. Further, assessing the role of Vitamin D levels on the shortening of TL can prove to be crucial biomarkers in managing optimal glycemic levels in T2DM patients.

The strengths of this study are the findings, which will help clinicians to predict the prognosis in patients precipitating with complications of DR, CVD, and coronary heart disease. The clinicians can then monitor complications aggressively to prescribing preventive drugs. Our study findings if replicated in further studies having a larger sample size, possibly it could guide policymakers and researchers to focus on preventive aspects. Since the observations in this study are cross-sectional. Therefore, we are unable to justify whether shortened TL is a trait marker or shortening halts with treatment. We have included patients only with T2DM. If this association exists in patients with T1DM, pancreatic disorders, or who had diabetes secondary to other systemic diseases or known cases of chronic inflammatory or infectious diseases needs to be assessed.

Acknowledgments

The authors would like to acknowledge all study participants.

Ethical clearance

Ethical clearance obtained from the Institutional Ethics committee (No. MDC/DOME/50).

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Cheng F, Carroll L, Joglekar MV, Januszewski AS, Wong KK, Hardikar AA, et al. Diabetes, metabolic disease, and telomere length. Lancet Diabetes Endocrinol 2021;9:117-26.  Back to cited text no. 1
    
2.
Zhao J, Miao K, Wang H, Ding H, Wang DW. Association between telomere length and type 2 diabetes mellitus: A meta-analysis. PLoS One 2013;8:e79993.  Back to cited text no. 2
    
3.
Asiimwe D, Mauti G, Kiconco R. Prevalence and risk factors associated with type 2 diabetes in elderly patients aged 45-80 years at Kanungu district. J Diabet Res 2019;2020:1-5.  Back to cited text no. 3
    
4.
Kumar PS, Vinapamula KS, Suchitra MM, Sachan A. Study on the association of Vitamin D with glycaemic control in patients with type 2 diabetes mellitus. J Clin Sci Res 2019;8:188-92.  Back to cited text no. 4
  [Full text]  
5.
Liu J, Cahoon E, Linet M, Little M, Dagnall C, Higson H, et al. Relationship between plasma 25-hydroxymitamin D and leucocyte telomere length by sex and race in a US study. Br J Nutr 2016;116:953-60.  Back to cited text no. 5
    
6.
Williams DM, Palaniswamy S, Sebert S, Buxton JL, Blakemore AI, Hyppönen E, et al. 25-hydroxyvitamin D concentration and leukocyte telomere length in young adults: Findings From the Northern Finland Birth Cohort 1966. Am J Epidemiol 2016;183:191-8.  Back to cited text no. 6
    
7.
Richards JB, Valdes AM, Gardner JP, Paximadas D, Kimura M, Nessa A, et al. Higher serum Vitamin D concentrations are associated with longer leukocyte telomere length in women. Am J Clin Nutr 2007;86:1420-5.  Back to cited text no. 7
    
8.
Pusceddu I, Farrell CJ, Di Pierro AM, Jani E, Herrmann W, Herrmann M. The role of telomeres and Vitamin D in cellular aging and age-related diseases. Clin Chem Lab Med 2015;53:1661-78.  Back to cited text no. 8
    
9.
Zhu H, Guo D, Li K, Pedersen-White J, Stallmann-Jorgensen IS, Huang Y, et al. Increased telomerase activity and Vitamin D supplementation in overweight African Americans. Int J Obes (Lond) 2012;36:805-9.  Back to cited text no. 9
    
10.
Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414:813-20.  Back to cited text no. 10
    
11.
American Diabetes Association. 2. Classification and diagnosis of diabetes: Standards of medical care in diabetes-2020. Diabetes Care 2020;43:S14-31.  Back to cited text no. 11
    
12.
Adaikalakoteswari A, Balasubramanyam M, Mohan V. Telomere shortening occurs in Asian Indian type 2 diabetic patients. Diabet Med 2005;22:1151-6.  Back to cited text no. 12
    
13.
Scherczinger CA, Bourke MT, Ladd C, Lee HC. DNA extraction from liquid blood using QIAamp. J Forensic Sci 1997;42:893-6.  Back to cited text no. 13
    
14.
O'Callaghan NJ, Fenech M. A quantitative PCR method for measuring absolute telomere length. Biol Proced Online 2011;13:3.  Back to cited text no. 14
    
15.
QIAGEN Supplementary Protocol: Isolation of Genomic DNA from Saliva and Mouthwash Using the QIAamp® DNA Blood Mini Kit; Spin Procedure. Available from: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rnapurification/dna-purification/genomic-dna/qiaamp-dna-mini-kit/#orderinginformation. [Last accessed on 2020 Nov 11].  Back to cited text no. 15
    
16.
Gallelli L, Michniewicz A, Cione E, Squillace A, Colosimo M, Pelaia C, et al. 25-hydroxy Vitamin D detection using different analytic methods in patients with migraine. J Clin Med 2019;8:895.  Back to cited text no. 16
    
17.
Wang J, Dong X, Cao L, Sun Y, Qiu Y, Zhang Y, et al. Association between telomere length and diabetes mellitus: A meta-analysis. J Int Med Res 2016;44:1156-73.  Back to cited text no. 17
    
18.
Baltzis D, Meimeti E, Grammatikopoulou MG, Roustit M, Mavrogonatou E, Kletsas D, et al. Assessment of telomerase activity in leukocytes of type 2 diabetes mellitus patients having or not foot ulcer: Possible correlation with other clinical parameters. Exp Ther Med 2018;15:3420-4.  Back to cited text no. 18
    
19.
Vasanthakumar J, Kambar S. Prevalence of obesity among type 2 diabetes mellitus patients in urban areas of Belagavi. Indian J Health Sci Biomed Res 2020;13:21-7.  Back to cited text no. 19
  [Full text]  
20.
Lee M, Martin H, Firpo MA, Demerath EW. Inverse association between adiposity and telomere length: The Fels Longitudinal Study. Am J Hum Biol 2011;23:100-6.  Back to cited text no. 20
    
21.
Patil RS, Gothankar JS. Assessment of risk of type 2 diabetes using the Indian Diabetes Risk Score in an urban slum of Pune, Maharashtra, India: A cross-sectional study. WHO South East Asia J Public Health 2016;5:53-61.  Back to cited text no. 21
    
22.
Arsenis NC, You T, Ogawa EF, Tinsley GM, Zuo L. Physical activity and telomere length: Impact of aging and potential mechanisms of action. Oncotarget 2017;8:45008-19.  Back to cited text no. 22
    
23.
Dave M, Gupta AK, Patel P, Heernath. Correlation between fasting blood sugar level, HbA1C level and serum lipid levels in type 2 diabetes mellitus patients. Int J Contemp Med Res 2019;6:G26-9.  Back to cited text no. 23
    
24.
Piplani S, Alemao NN, Prabhu M, Ambar S, Chugh Y, Chugh SK. Correlation of the telomere length with type 2 diabetes mellitus in patients with ischemic heart disease. Indian Heart J 2018;70 Suppl 3:S173-6.  Back to cited text no. 24
    
25.
Bamanikar SA, Bamanikar AA, Arora A. Study of serum urea and creatinine in diabetic and non-diabetic patients in in a tertiary teaching hospital. J Med Res 2016;2:12-5.  Back to cited text no. 25
    
26.
Kostoglou-Athanassiou I, Athanassiou P, Gkountouvas A, Kaldrymides P. Vitamin D and glycemic control in diabetes mellitus type 2. Ther Adv Endocrinol Metab 2013;4:122-8.  Back to cited text no. 26
    
27.
Kavadar G, Demircioğlu DT, Özgönenel L, Emre TY. The relationship between Vitamin D status, physical activity and insulin resistance in overweight and obese subjects. Bosn J Basic Med Sci 2015;15:62-6.  Back to cited text no. 27
    
28.
Xu X, Hu H, Lin Y, Huang F, Ji H, Li Y, et al. Differences in leukocyte telomere length between coronary heart disease and normal population: A multipopulation meta-analysis. Biomed Res Int 2019;2019:5046867.  Back to cited text no. 28
    
29.
Sharma R, Gupta A, Thungapathra M, Bansal R. Telomere mean length in patients with diabetic retinopathy. Sci Rep 2015;5:18368.  Back to cited text no. 29
    
30.
Luo BA, Gao F, Qin LL. The association between Vitamin D deficiency and diabetic retinopathy in type 2 diabetes: A meta-analysis of observational studies. Nutrients 2017;9:307.  Back to cited text no. 30
    
31.
Alaidarous TA, Alkahtani NM, Aljuraiban GS, Abulmeaty MM. Impact of the glycemic control and duration of type 2 diabetes on Vitamin D level and cardiovascular disease risk. J Diabetes Res 2020;2020:8431976.  Back to cited text no. 31
    
32.
Dudinskaya EN, Tkacheva ON, Shestakova MV, Brailova NV, Strazhesko ID, Akasheva DU, et al. Short telomere length is associated with arterial aging in patients with type 2 diabetes mellitus. Endocr Connect 2015;4:136-43.  Back to cited text no. 32
    
33.
Kumarchandra R, Sudha K, Ahmad A. Vitamin D status: A link between blood glucose and renal function indicators. Int J Pharm Bio Sci 2016;7:734-8.  Back to cited text no. 33
    
34.
Rezende PC, Hlatky MA, Hueb W, Garcia RM, da Silva Selistre L, Lima EG, et al. Association of longitudinal values of glycated hemoglobin with cardiovascular events in patients with type 2 diabetes and multivessel coronary artery disease. JAMA Netw Open 2020;3:e1919666.  Back to cited text no. 34
    
35.
Ul-Haque I, Ud Deen Z, Shafique S, Ur Rehman SI, Zaman M, Basalat ST, et al. The role of glycated hemoglobin A1c in determining the severity of coronary artery disease in diabetic and non-diabetic subjects in Karachi. Cureus 2019;11:e4982.  Back to cited text no. 35
    
36.
Babikr WG, Alshahrani AS, Hamid HG, Abdelraheem AH, Shalayel MH. The correlation of HbA1c with body mass index and HDL-cholesterol in type 2 diabetic patients. Biomed Res 2016;27:1280-3.  Back to cited text no. 36
    
37.
Ketema EB, Kibret KT. Correlation of fasting and postprandial plasma glucose with HbA1c in assessing glycemic control; systematic review and meta-analysis. Arch Public Health 2015;73:43.  Back to cited text no. 37
    
38.
Mauss D, Jarczok MN, Hoffmann K, Thomas GN, Fischer JE. Association of Vitamin D levels with type 2 diabetes in older working adults. Int J Med Sci 2015;12:362-8.  Back to cited text no. 38
    


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